The Biology Project > Biomath > Linear Functions > Applications > Enzyme Kinetics

Linear Functions Applications

Enzyme Kinetics

Problem 3- Given a Lineweaver-Burk plot, determine the Vmax of a particular enzyme.

The following is a Lineweaver-Burk plot of kinetic data for a particular enzyme. Based on this data, determine the Vmax of the enzyme.

graph 1/[S] vs 1/V

Recall the Lineweaver-Burk equation,

1/V = (Km + [S])/Vmax[S] = (km/vmax)(1/[S]) + 1/vmax.

 

 

 

 

 

 

A.

 

 0.04 µM

B.

 

 0.02 µM/sec

C.

 

 -25 µM

D.

 

 50 µM/sec

E.

 

 0.5 1/sec

F.

 

 2 sec

Problem 4- Given a Lineweaver-Burk plot, determine the Km of a particular enzyme.

The Biology Project > Biomath > Linear Functions > Applications > Enzyme Kinetics

The Biology Project
Department of Biochemistry and Molecular Biophysics
The University of Arizona
May 2006
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