Linear Functions Applications
Enzyme Kinetics
Problem 4- Given a Lineweaver-Burk plot, determine the Km of a particular enzyme.
The following is a Lineweaver-Burk plot of kinetic data for a particular enzyme. Based on this data, determine the Km of the enzyme.
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![graph of 1/[s] vs 1/V](graphics/EnzKinetfig1.gif)
![1/V = (Km + [S])/Vmax[S] = (km/vmax)(1/[S]) + 1/vmax.](graphics/EnzymeKineticsequn.gif)
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0.01 µM |
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-0.04 µM |
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0.02 µM |
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50 µM |
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0.5 µM |
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25 µM |
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-0.5 µM |
Problem 5- Determine the 1/[S]-intercept of the Lineweaver-Burk equation.
The Biology Project > Biomath > Linear Functions > Applications > Enzyme Kinetics
Department of Biochemistry and Molecular Biophysics
The University of Arizona
May 2006
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