DNA fingerprint analysis is based on the "Southern" hybridization technique. In this method:

A.	Nonradioactive cellular RNA, separated by electrophoresis and blotted onto a membrane filter, is tested for hybridization with a radioactive gene-specific probe of either RNA or DNA.
B.	Radioactive DNA restriction fragments, separated by electrophoresis and blotted onto a membrane filter are tested for hybridization with a non-radioactive, gene-specific probe of either RNA or DNA.
C.	Radioactive cellular RNA, separated by electrophoresis and blotted onto a membrane filter is tested for hybridization with a radioactive, gene-specific probe of either RNA or DNA.
D.	DNA research is done south of the equator.
E.	Nonradioactive DNA restriction fragments, separated by electrophoresis and blotted onto a membrane filter are tested for hybridization with a radioactive, gene-specific probe of either RNA or DNA. Restriction endonuclease digestion of a sample of human DNA can yield more than a million different fragments. The essence of the RFLP method is the use of a "single locus" probe, normally radioactively labeled, to detect a locus of the human genome with alleles that are highly variable.

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The Biology Project
University of Arizona
Tuesday, August 20, 1996
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