Metabolism Problem Set
Problem 5 Tutorial: Effect of pH on Mitochondria
If you isolate mitochondria and place them in buffer with a low pH they begin to manufacture ATP. Why?
Permeability of outer membrane
Mitochondrial production of ATP requires a concentration gradient of H+, with a high concentration at the inter membrane space and a low concentration in the matrix. The inner membrane is is impermeable to H+, but the outer membrane of the mitochondria will allow H+ to pass through.
Thus, placing mitochondria in a low pH buffer produces a H+ gradient that can generate ATP through ATP synthetase.
The Biology Project
University of Arizona
Tuesday, August 20, 1996
Contact the Development Team
All contents copyright © 1996. All rights reserved.