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Construct a standard
curve
To begin, measure the migration distance (in centimeters) from the bottom of
the well to the bottom of the band for each band for the size standard. In
tabular form the migration distance for the size standards in relationship to
size of DNA (in base pairs) is:
| Distance migrated (cm) |
Size (bp) | Log
MW | | 0.3 |
10,000 | 4.00 | | 0.7 |
8,000 | 3.90 | | 1.1 |
6,000 | 3.78 | | 1.5 |
5,000 | 3.70 | | 1.9 |
4,000 | 3.60 | | 2.6 |
3,000 | 3.48 | | 3.5 |
2,000 | 3.30 | | 4.2 |
1,500 | 3.18 | | 5.2 |
1,000 | 3.00 | | 6.7 |
500 | 2.70 |
For the example above the following standard curve can be created.
Introduction | Step 2
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