ELISA Activity

Question 3

What would happen if the anti-human Ig-conjugate were not washed free of the well before the substrate was added?
All wells would show uniform overdevelopment due to unbound and excess anti-human Ig enzyme conjugate.
Since the enzyme which acts on the substrate is in excess, it would turn all the wells a uniform color whether they were truly positive or not.

The Biology Project
University of Arizona
Monday, March 31, 1997

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