Recombinant DNA Technology Problem Set

Problem 2 Recombinant DNA 1


Which of the following tools of recombinant DNA technology is INCORRECTLY paired with one of its uses?

A. restriction endonuclease - production of DNA fragments for gene cloning.
B. DNA ligase - enzyme that cuts DNA, creating sticky ends.
Yes, DNA ligase joins adjacent nucleotides in a covalent linkage. Restriction endonucleases cut DNA at specific sites creating sticky ends.
C. DNA polymerase - copies DNA sequences in the polymerase chain reaction.
D. reverse transcriptase - production of cDNA from mRNA.
E. electrophoresis - RLFP analysis.

The Biology Project
University of Arizona
Tuesday, October 29, 1996
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