Recombinant DNA Technology Problem Set

Problem 4: Recombinant DNA 2

Tutorial to help answer the question

DNA from a eukaryotic organism is digested with a restriction endonuclease and the resulting fragments cloned into a plasmid vector. Bacteria transformed by these plasmids collectively contain all of the genes of the organism. This culture of bacteria is refereed to as a:


Gene library

When the genomic DNA is digested by a restriction endonuclease, and all fragments cloned at random into a plasmid vector, then the majority of genetic information will be included in the mixture of bacteria.

Cultures of the bacteria, with each containing only a fraction of the genome, collectively contain all the genes and are called a library.

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Updated: July 15, 1999
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