Recombinant DNA Technology Problem Set

Problem 5 Recombinant DNA 3

Which of the following is not part of the normal process of cloning recombinant DNA in bacteria?

A. restriction endonuclease digestion of cellular and plasmid DNAs.
B. production of recombinant DNA using DNA ligase and a mixture of digested cellular and plasmid DNAs.
C. separation of recombinant DNAs by electrophoresis using the Southern technique to determine where the desired recombinant migrates.
D. transformation of bacteria by the recombinant DNA plasmids and selection using ampicillin .
E. probing blots of bacteria clones with radioactive DNA complementary to the desired gene.

The Biology Project
University of Arizona
Tuesday, October 29, 1996
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