Recombinant DNA Technology Problem Set

Problem 11 Identifying recombinants

A key feature of insertional mutagenesis for the identification of plasmids containing recombinant DNA is:

A. the production of nutritional auxotrophs.
B. the DNA sequencing of recombinant plasmids.
C. the production of restriction endonuclease maps of recombinant plasmids.
D. introns can be moved to new locations within the gene.
E. the disruption of a gene on the plasmid by the inserted recombinant DNA.
For example, ß-galactosidase on the plasmid can be disrupted by the inserted gene.

The Biology Project
University of Arizona
Tuesday, October 29, 1996
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