Molecular Genetics of Prokaryotes Problem Set
Problem 10: Phenotype of cells diploid for lac I geneTutorial to help answer the question
An E. coli strain that is lac I- is conjugated with E. coli cells carrying an F' plasmid with the PI-lac I+ DNA sequence on the episome. How would the expression of the lac Z+ be regulated in the resulting cells that are diploid and heterozygous for the lac I gene (lac I+ on episome and lac I- on the bacterial chromosome)?
Notice that the creation of a partial diploid cell line, containing both lac I+ and lac I- alleles can be used to demonstrate that the lac I+ allele is dominant.
University of Arizona
Revised: November 14, 2000
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