Nucleic Acids and the Genetic Material Problem Set 1
Problem 5: More on the replication fork
In this diagram of the process of DNA replication at a replication fork, the black boxes labeled D and E are:
- DNA template with synthesis of a new strand in the 5' to 3' direction.
- The 5' triphosphate can only be added to a free 3'OH of deoxyribose.
- The two antiparallel strands are replicated simultaneously in both directions.
- RNA primers are used to initiate a new strand.
- The parent strand at the 3' end of the template determines the
daughter or leading strand in continuous replication.
- The parent strand at the 5' end of the template produces the lagging
strand as short pieces of DNA (100-200 nucleotides in eukaryotes and longer
- The lagging strand fragments are called Okazaki fragments after
their discoverer, Reiji Okazaki.
- The RNA primers are removed by DNA polymerase and the fragments are
joined by DNA ligase.
Direction of replication
The replication fork
University of Arizona
Thursday, October 3, 1996
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