In collaboration with Dr. Evan Hersh and Dr. Doug Lake at the Arizona Cancer Center
and the Department of Microbiology and Immunology, we have been studying antibodies
produced by B-cells that infiltrate human tumors. The exciting observation is that
many human tumors include B-cells that secrete anti-tumor antibody in vitro. Using
methods developed by Doug Lake, a graduate student Hua Zhang was able to amplify,
clone, and express a fragment of antitumor antibody (AZM1) that shows specificity for
melanoma cells. We are pursuing studies on the nature of the antigen recognized and
are also isolating B-cells from other kinds of cancer.
Punt, C.J.A., J.A.M. Barbuto, W.J. Grimes., and E.M. Hersh. "Antitumor Antibody
Produced by Human Tumor-infiltrating and Peripheral Blood B Lymphocytes."
Cancer Imm. Immunother. 38, 225-231 (1994)
Barbuto, J.A., Grimes, W.J. and E.M. Hersh." Antibodies to TNF: a component of
B-cell immune responses with a role in tumor-host interaction." Cancer Imm.
Immunother. 40, 31-36 (1995)
Zhang, H., Lake, D.F., Barbuto, J.A., Bernstein, R.M., Grimes, W.J., and Hersh, E.M.
"A Human Monoclonal Anti-melanoma Single Chain Fv(scFv) Antibody Derived from Tumor
Infiltrating Lymphocytes." Cancer Research 55, 3584-3591 (1995)